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gblocks fragment containing 5 sgrna expression cassettes with high fidelity four-base overhang pair  (GenScript corporation)

 
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    GenScript corporation gblocks fragment containing 5 sgrna expression cassettes with high fidelity four-base overhang pair
    a, Framework for converting TAG codons into TAA in human cells. b, UAGs number and editable UAG sites of all genes and essential genes in each chromosome. c-e, distribution analysis of cells with different number of modified gene targets in populations with different delivery methods based on single cell RNAseq. Method_1, delivery 10 <t>gBlocks</t> with mCherry-inactivated eGFP reporter; Method_2, delivery 10 gBlocks with mCherry-inactivated eGFP reporter and <t>eGFP</t> <t>sgRNA</t> plasmids; Method_3, delivery 43-all-in-one with DsRed. f, Density plot for distribution of number of modified gene targets detected by scRNAseq in 3 populations. Vertical lines indicate the median values of modified gene targets. g, For each gene target, distribution analysis of modified cells with different editing efficiency. Counts from different methods were stacked in the plot. h, Editing efficiency of each sgRNAs in single cells. i, Heat map of target “C” editing efficiency in cell population with three delivery methods based on converting single-cell RNA-Seq into Bulk RNA-Seq. Editing efficiency was indicated with the intensity of red.
    Gblocks Fragment Containing 5 Sgrna Expression Cassettes With High Fidelity Four Base Overhang Pair, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gblocks+fragment+containing+5+sgrna+expression+cassettes+with+high+fidelity+four-base+overhang+pair/bio_rxiv__2021__07__13__452007-96-1-37?v=GenScript+corporation
    Average 90 stars, based on 1 article reviews
    gblocks fragment containing 5 sgrna expression cassettes with high fidelity four-base overhang pair - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "Multiplex base editing to convert TAG into TAA codons in the human genome"

    Article Title: Multiplex base editing to convert TAG into TAA codons in the human genome

    Journal: bioRxiv

    doi: 10.1101/2021.07.13.452007

    a, Framework for converting TAG codons into TAA in human cells. b, UAGs number and editable UAG sites of all genes and essential genes in each chromosome. c-e, distribution analysis of cells with different number of modified gene targets in populations with different delivery methods based on single cell RNAseq. Method_1, delivery 10 gBlocks with mCherry-inactivated eGFP reporter; Method_2, delivery 10 gBlocks with mCherry-inactivated eGFP reporter and eGFP sgRNA plasmids; Method_3, delivery 43-all-in-one with DsRed. f, Density plot for distribution of number of modified gene targets detected by scRNAseq in 3 populations. Vertical lines indicate the median values of modified gene targets. g, For each gene target, distribution analysis of modified cells with different editing efficiency. Counts from different methods were stacked in the plot. h, Editing efficiency of each sgRNAs in single cells. i, Heat map of target “C” editing efficiency in cell population with three delivery methods based on converting single-cell RNA-Seq into Bulk RNA-Seq. Editing efficiency was indicated with the intensity of red.
    Figure Legend Snippet: a, Framework for converting TAG codons into TAA in human cells. b, UAGs number and editable UAG sites of all genes and essential genes in each chromosome. c-e, distribution analysis of cells with different number of modified gene targets in populations with different delivery methods based on single cell RNAseq. Method_1, delivery 10 gBlocks with mCherry-inactivated eGFP reporter; Method_2, delivery 10 gBlocks with mCherry-inactivated eGFP reporter and eGFP sgRNA plasmids; Method_3, delivery 43-all-in-one with DsRed. f, Density plot for distribution of number of modified gene targets detected by scRNAseq in 3 populations. Vertical lines indicate the median values of modified gene targets. g, For each gene target, distribution analysis of modified cells with different editing efficiency. Counts from different methods were stacked in the plot. h, Editing efficiency of each sgRNAs in single cells. i, Heat map of target “C” editing efficiency in cell population with three delivery methods based on converting single-cell RNA-Seq into Bulk RNA-Seq. Editing efficiency was indicated with the intensity of red.

    Techniques Used: Modification, RNA Sequencing



    Similar Products

    gblocks fragment containing 5 sgrna expression cassettes with high fidelity four-base overhang pair  (GenScript corporation)
    90
    GenScript corporation gblocks fragment containing 5 sgrna expression cassettes with high fidelity four-base overhang pair
    a, Framework for converting TAG codons into TAA in human cells. b, UAGs number and editable UAG sites of all genes and essential genes in each chromosome. c-e, distribution analysis of cells with different number of modified gene targets in populations with different delivery methods based on single cell RNAseq. Method_1, delivery 10 <t>gBlocks</t> with mCherry-inactivated eGFP reporter; Method_2, delivery 10 gBlocks with mCherry-inactivated eGFP reporter and <t>eGFP</t> <t>sgRNA</t> plasmids; Method_3, delivery 43-all-in-one with DsRed. f, Density plot for distribution of number of modified gene targets detected by scRNAseq in 3 populations. Vertical lines indicate the median values of modified gene targets. g, For each gene target, distribution analysis of modified cells with different editing efficiency. Counts from different methods were stacked in the plot. h, Editing efficiency of each sgRNAs in single cells. i, Heat map of target “C” editing efficiency in cell population with three delivery methods based on converting single-cell RNA-Seq into Bulk RNA-Seq. Editing efficiency was indicated with the intensity of red.
    Gblocks Fragment Containing 5 Sgrna Expression Cassettes With High Fidelity Four Base Overhang Pair, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gblocks+fragment+containing+5+sgrna+expression+cassettes+with+high+fidelity+four-base+overhang+pair/bio_rxiv__2021__07__13__452007-96-1-37?v=GenScript+corporation
    Average 90 stars, based on 1 article reviews
    gblocks fragment containing 5 sgrna expression cassettes with high fidelity four-base overhang pair - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

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    a, Framework for converting TAG codons into TAA in human cells. b, UAGs number and editable UAG sites of all genes and essential genes in each chromosome. c-e, distribution analysis of cells with different number of modified gene targets in populations with different delivery methods based on single cell RNAseq. Method_1, delivery 10 gBlocks with mCherry-inactivated eGFP reporter; Method_2, delivery 10 gBlocks with mCherry-inactivated eGFP reporter and eGFP sgRNA plasmids; Method_3, delivery 43-all-in-one with DsRed. f, Density plot for distribution of number of modified gene targets detected by scRNAseq in 3 populations. Vertical lines indicate the median values of modified gene targets. g, For each gene target, distribution analysis of modified cells with different editing efficiency. Counts from different methods were stacked in the plot. h, Editing efficiency of each sgRNAs in single cells. i, Heat map of target “C” editing efficiency in cell population with three delivery methods based on converting single-cell RNA-Seq into Bulk RNA-Seq. Editing efficiency was indicated with the intensity of red.

    Journal: bioRxiv

    Article Title: Multiplex base editing to convert TAG into TAA codons in the human genome

    doi: 10.1101/2021.07.13.452007

    Figure Lengend Snippet: a, Framework for converting TAG codons into TAA in human cells. b, UAGs number and editable UAG sites of all genes and essential genes in each chromosome. c-e, distribution analysis of cells with different number of modified gene targets in populations with different delivery methods based on single cell RNAseq. Method_1, delivery 10 gBlocks with mCherry-inactivated eGFP reporter; Method_2, delivery 10 gBlocks with mCherry-inactivated eGFP reporter and eGFP sgRNA plasmids; Method_3, delivery 43-all-in-one with DsRed. f, Density plot for distribution of number of modified gene targets detected by scRNAseq in 3 populations. Vertical lines indicate the median values of modified gene targets. g, For each gene target, distribution analysis of modified cells with different editing efficiency. Counts from different methods were stacked in the plot. h, Editing efficiency of each sgRNAs in single cells. i, Heat map of target “C” editing efficiency in cell population with three delivery methods based on converting single-cell RNA-Seq into Bulk RNA-Seq. Editing efficiency was indicated with the intensity of red.

    Article Snippet: All gBlocks fragment containing 5 sgRNA expression cassettes with high fidelity four-base overhang pair after cutting with type IIS restriction enzyme BbsI restriction enzyme were designed and directly sent to be synthesized into PUC57 cloning plasmid by GenScript.

    Techniques: Modification, RNA Sequencing